| Product Name | c-Myc antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal [9E11] to c-MycThe c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment. |
| Immunogen | Synthetic peptide: AEEQKLISEEDL conjugated to KLH, corresponding to amino acids 408-420 of Human c-Myc. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 9E11 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FIHC-PIPWBChIP |
| Suggested working dilutions: Immunoprecipitation: 2 µg/mg protein lysate Western blotting: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the end user: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | c Myc 4609 P01106 MYC cMyc 113970 c-Myc |
|---|---|
| Target | c-Myc |
| Cellular Localization | Nuclear and Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | HL-60 cells or breast tumors. |
|---|---|
| Application Notes | Suggested working dilutions: Immunoprecipitation: 2 µg/mg protein lysate Western blotting: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the end user: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4¼C short term. For extended storage aliquot and store at -20¼C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline |
