Application
Sample (30 ug of whole cell lysate) A: SK-N-SH 12% SDS PAGE diluted at 1:500Application
CNOT8 antibody detects CNOT8 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CNOT8 protein stained by CNOT8 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | CNOT8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CNOT8 (CCR4-NOT transcription complex, subunit 8) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CNOT8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (99%), Rat (100%), Zebrafish (87%), Xenopus laevis (95%), Chicken (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAF1 antibody CALIF antibody POP2 antibody hCAF1 antibody CNOT8 antibody CCR4-NOT transcription complex subunit 8 antibody CALIFp antibody CCR4-associated factor 8 antibody CAF1-like protein antibody PGK promoter directed over production antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (7) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: SK-N-SH 12% SDS PAGE diluted at 1:500
Application
CNOT8 antibody detects CNOT8 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CNOT8 protein stained by CNOT8 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Positive Control | SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
