Application
Bovine brain extract was separated on SDS-PAGE and probed with Monoclonal AB To CNP-ASE (MO) CL:11-5B (RB) (Cat. No. ). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBT/BCIP substrate. Antibody dilution 1/1000| Product Name | CNPase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [11 - 5B] to CNPase - Oligodendrocyte MarkerCNPase (2',3'-Cyclic Nucleotide 3'-Phosphodiesterase) exists in two forms: 46kDa (CNP 1) and 48kDa (CNP 2). It is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system and is virtual absent in other celltypes. CNPase activity is decreased in demyelinating diseases such as multiple sclerosis. CNPase gene is localized to chromosome 17q21, very near the BRCA 1 familial breast cancer gene. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 11-5B |
| Host Species | Mouse |
| Tested Applications | ConjugationDotELISAICC/IFIHCIHC-FIHC-PIPWB |
| Conjugation: Use at an assay dependant dilution. Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/500 for immunoblotting of fresh bovine whole brain extract Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogPigRatRabbitSheepBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P09543 CNPase 123830 1267 CNP1 CNP |
|---|---|
| Target | CNPase |
| Tissue Specificity | Reacts specifically with CNPase. In an immunoblotting assay, the antibody localizes both CNP1 (46kD) and CNP2 (48kD) bands.Immunohistochemical staining of paraffin, cryostat or vibratome sections of rat brain reveals selective staining of oligodendrocytes in the grey and white matter. Nerve cells and axons are not stained and astroglial cells do not appear to be labelled. |
Application
Bovine brain extract was separated on SDS-PAGE and probed with Monoclonal AB To CNP-ASE (MO) CL:11-5B (RB) (Cat. No. ). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBT/BCIP substrate. Antibody dilution 1/1000| Positive Control | brain sections |
|---|---|
| Application Notes | Conjugation: Use at an assay dependant dilution. Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/500 for immunoblotting of fresh bovine whole brain extract Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Ascites fluid |
