Application
Sample (30μg whole cell lysate) A:HeLa S3 7.5% SDS PAGE diluted at 1:1000Application
CoA synthase antibody [N3C3-3] detects CoA synthase protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CoA synthase antibody [N3C3-3] diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CoA synthase antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using CoA synthase antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:3000Application
CoA synthase antibody [N3C3-3] detects CoA synthase protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. CoA synthase antibody [N3C3-3] diluted at 1:500.| Product Name | CoA synthase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CoA synthase (Coenzyme A synthase)Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CoA synthase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, PigRat (85%), Pig (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DPCK antibody FLJ35179 antibody NBP antibody PPAT antibody UKR1 antibody pOV-2 antibody COASY antibody phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody bifunctional coenzyme A synthase antibody nucleotide binding protei |
|---|---|
| Molecular Weight(MW) | 62 kDa (note) (6) |
| Cellular Localization | Cytoplasm |
Application
Sample (30μg whole cell lysate) A:HeLa S3 7.5% SDS PAGE diluted at 1:1000
Application
CoA synthase antibody [N3C3-3] detects CoA synthase protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CoA synthase antibody [N3C3-3] diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CoA synthase antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using CoA synthase antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:3000
Application
CoA synthase antibody [N3C3-3] detects CoA synthase protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. CoA synthase antibody [N3C3-3] diluted at 1:500.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
