Application
Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed H1299, using COBRA1 antibody at 1:500 dilution.Application
COBRA1 antibody detects COBRA1 protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. COBRA1 antibody dilution: 1:500.Application
COBRA1 antibody detects COBRA1 protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. COBRA1 antibody dilution: 1:500.Application
COBRA1 antibody detects COBRA1 protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. COBRA1 antibody dilution: 1:500.| Product Name | COBRA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COBRA1 (cofactor of BRCA1)NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COBRA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, ChickenRat (99%), Zebrafish (86%), Xenopus laevis (85%), Pig (97%), Chicken (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp586B0519 antibody KIAA1182 antibody NELF-B antibody NELFB antibody COBRA1 antibody negative elongation factor protein B antibody negative elongation factor B antibody cofactor of BRCA1 antibody |
|---|---|
| Molecular Weight(MW) | 66 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed H1299, using COBRA1 antibody at 1:500 dilution.
Application
COBRA1 antibody detects COBRA1 protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. COBRA1 antibody dilution: 1:500.
Application
COBRA1 antibody detects COBRA1 protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. COBRA1 antibody dilution: 1:500.
Application
COBRA1 antibody detects COBRA1 protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. COBRA1 antibody dilution: 1:500.| Positive Control | H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
