Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] diluted at 1:1000.Application
Immunoprecipitation of Cofilin 1 protein from 293T whole cell extracts using 5 μg of Cofilin 1 antibody [GT217] . Western blot analysis was performed using Cofilin 1 antibody [GT217] . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin 1 antibody [GT217] diluted at 1:250.Application
Cofilin 1 antibody [GT217] detects Cofilin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] diluted at 1:1000.| Product Name | Cofilin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT217] to Cofilin 1 (cofilin 1 (non-muscle))Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT217 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Sheep, Rhesus Monkey, BovineRat (99%), Pig (99%), Sheep (99%), Rhesus Monkey (99%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CFL1 CFL cofilin 1 (non-muscle) |
|---|---|
| Target | Cofilin 1 |
| Molecular Weight(MW) | 19 kDa (note) |
| Cellular Localization | Nucleus matrix , Cytoplasm , cytoskeleton |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] diluted at 1:1000.
Application
Immunoprecipitation of Cofilin 1 protein from 293T whole cell extracts using 5 μg of Cofilin 1 antibody [GT217] . Western blot analysis was performed using Cofilin 1 antibody [GT217] . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin 1 antibody [GT217] diluted at 1:250.
Application
Cofilin 1 antibody [GT217] detects Cofilin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] diluted at 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
