Application
Sample (20 ug of whole cell lysate) A: HeLa membrane 7.5% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COG7 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | COG7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COG7 (component of oligomeric golgi complex 7)Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG7 (Ungar et al., 2002 [PubMed 11980916]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human COG7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, BovineMouse (97%), Rat (96%), Zebrafish (81%), Xenopus laevis (84%), Chicken (82%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDG2E antibody COG7 antibody conserved oligomeric Golgi complex subunit 7 antibody COG complex subunit 7 antibody component of oligomeric golgi complex 7 antibody |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) |
| Cellular Localization | Golgi apparatus membrane |
Application
Sample (20 ug of whole cell lysate) A: HeLa membrane 7.5% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COG7 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | HeLa mf |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
