Application
Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 μg of Coilin antibody . Western blot analysis was performed using Coilin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Coilin antibody detects Coilin protein at nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. Coilin antibody diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Coilin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.| Product Name | Coilin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Coilin (coilin)The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Coilin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CLN80 antibody p80-coilin antibody COIL antibody coilin antibody coilin p80 antibody p80 antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa (note) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 μg of Coilin antibody . Western blot analysis was performed using Coilin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Coilin antibody detects Coilin protein at nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. Coilin antibody diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Coilin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
