Application
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Polyethylene glycol tert-octylphenyl ether. Fixed cells were stained with 10 μg/mL Monoclonal Anti-Coilin Clone: pδ . The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.| Product Name | Coilin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [Pdelta] to CoilinThe description of specific intranuclear structures known today as Cajal bodies was first published in 1903 by the neuro-cytologist Ramon-y-Cajal. He observed that neurons stained with silver contained spherical structures of around 0.5 micron in diameter that were often associated with nucleoli and called them nucleolar accessory bodies. Later, the same bodies were called coiled bodies since when these structures were viewed by electron microscopy, they resembled a tangle of coiled threads. It was found that patients with autoantibodies against coiled bodies recognize a protein of 80 kDa termed p80-coilin. Using these antibodies, coiled bodies were identified in plants, flies, frogs, birds, and mammals. The gene encoding p80-coilin has been cloned and sequenced. It contains two nuclear localization sequences (NLS) (at amino acid 107-112 and 181-198) and several serine residues that are phosphorylated in vivo. Mutating Serine-202 to Aspartate causes the disappearance of coiled bodies and a redistribution of coilin to intranucleolar domains. Nuclear antigens shown to colocalize with p80 coilin in Cajal bodies include basal transcription factors, cell cycle factors (cdks), splicing snRNPs and nucleolar factors including snoRNPs. |
| Immunogen | Recombinant fragment (C-terminus of Human coilin, 389 amino acids). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | Pdelta |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | p80-coilin P38432 CLN80 COIL p80coilin 600272 Coilin 8161 p80 coilin |
|---|---|
| Target | Coilin |
| Cellular Localization | Nuclear |
Application
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Polyethylene glycol tert-octylphenyl ether. Fixed cells were stained with 10 μg/mL Monoclonal Anti-Coilin Clone: pδ . The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.| Positive Control | HeLa nuclear extract |
|---|---|
| Application Notes | ICC: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
