Application
Sample (50 ug of whole cell lysate) A: Mouse white adipose 7.5% SDS PAGE diluted at 1:1000Application
COL4A3BP antibody [C3], C-term detects COL4A3BP protein at cytoplasm on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. COL4A3BP antibody [C3], C-term dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed A431, using CERT antibody at 1:200 dilution.Application
Sample(30 μg of whole cell lysate) A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE diluted at 1:500| Product Name | COL4A3BP antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COL4A3BP (collagen, type IV, alpha 3 (Goodpasture antigen) binding protein)This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COL4A3BP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CERT antibody CERTL antibody FLJ20597 antibody GPBP antibody STARD11 antibody COL4A3BP antibody hCERT antibody StAR-related lipid transfer (START) domain containing 11 antibody ceramide transfer protein antibody collagen type IV alpha-3-binding pr |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Golgi apparatus , Endoplasmic reticulum |
Application
Sample (50 ug of whole cell lysate) A: Mouse white adipose 7.5% SDS PAGE diluted at 1:1000
Application
COL4A3BP antibody [C3], C-term detects COL4A3BP protein at cytoplasm on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. COL4A3BP antibody [C3], C-term dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed A431, using CERT antibody at 1:200 dilution.
Application
Sample(30 μg of whole cell lysate) A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE diluted at 1:500| Positive Control | HepG2 , Molt-4 , Raji , mouse white adipose tissue |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
