| Product Name | Collagen I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Collagen ICollagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This preparation results in a native conformation of the protein.Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Mutations in the gene encoding collagen 1 are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. |
| Immunogen | Collagen type I extracted and purified from mouse skin. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PRIA |
| ICC/IF:1:100~1:500 IHC:1:50~1:200 ELISA:1:5000~1:20000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | Cola2 AA960264 Col1a2 Cola-2 Mov13 AI325291 Col1a-1 oim 12843 Col1a1 Col1a-2 Cola-1 Cola1 Mov-13 Collagen I 12842 Cola 2 Cola 1 Mov 13 Col1a 1 Col1a 2 |
|---|---|
| Target | Collagen I |
| Tissue Specificity | This antibody is specific to Mouse Collagen Type I (100%) by RIA, and typically showed less than 1% reactivity against other types of mouse collagen types (II, III and IV). |
| Positive Control | Frozen mouse liver for IF |
|---|---|
| Application Notes | ICC/IF:1:100~1:500 IHC:1:50~1:200 ELISA:1:5000~1:20000 |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
