| Product Name | Collagen I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [C11 (2F2/51)] to Collagen IThis gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| Immunogen | Human collagen type I |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | C11 (2F2/51) |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-F |
| Immunohistology - Frozen: 10 µg/ml - 20 µg/ml Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | collagen type I alpha 2 COL1A2 OI4 |
|---|---|
| Target | Collagen I |
| Tissue Specificity | This antibody reacts with both the native and heat denatured forms. No cross reactivity was observed with albumin fractions of human serum or albumins of other species. Less than 5% binding was observed with collagen II in various assays. |
| Application Notes | Immunohistology - Frozen: 10 µg/ml - 20 µg/ml Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Prior to reconstitution store at +4°C. After reconstitution store at -20°C. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
