Application
Application| Product Name | Collagen V antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MC5-HA] to Collagen VType V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | MC5-HA |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-FRIAWB |
| ELISA: 1/1000. IHC-Fr: 1/20 if FITC-labeled secondary antibodies are used. Otherwise use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: ~180 kDa. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | 1290 MGC105115 COL5A3 COL5A2 P20908 COL5A1 120215 1289 P05997 P25940 50509 Collagen V 120216 120190 |
|---|---|
| Target | Collagen V |
| Tissue Specificity | reacts with native human as well as heat denatured human collagen type V in RIA, ELISA and immunoblot/WB assays. Does not react with other collagen types I, II, III, and IV. Human plasma proteins do not interfere with binding to collagen. |
| Cellular Localization | Cytoplasmic |
Application
Application| Application Notes | ELISA: 1/1000. IHC-Fr: 1/20 if FITC-labeled secondary antibodies are used. Otherwise use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: ~180 kDa. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
