Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:1000Application
COMT antibody detects COMT protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. COMT antibody dilution: 1:250.Application
COMT antibody detects COMT protein by Western blot analysis. A. 50 µg mouse spleen lysate/extract 12 % SDS-PAGE COMT antibody dilution: 1:500Application
ELISA detection of COMT using for capture at a concentration of 2.5 µg/mL and for detection at a concentration of 0.5 µg/mL.| Product Name | COMT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COMT (catechol-O-methyltransferase)Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COMT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rhesus Monkey, BovineRat (82%), Dog (81%), Pig (82%), Rhesus Monkey (93%), Bovine (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | COMT antibody catechol O-methyltransferase antibody catechol-O-methyltransferase antibody |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (4) |
| Cellular Localization | Isoform Soluble: Cytoplasm , Isoform Membrane-bound: Cell membrane |
Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:1000
Application
COMT antibody detects COMT protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. COMT antibody dilution: 1:250.
Application
COMT antibody detects COMT protein by Western blot analysis. A. 50 µg mouse spleen lysate/extract 12 % SDS-PAGE COMT antibody dilution: 1:500
Application
ELISA detection of COMT using for capture at a concentration of 2.5 µg/mL and for detection at a concentration of 0.5 µg/mL.| Positive Control | Molt-4 , mouse spleen |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
