Application
ICC/IF analysis of HepG2 cells using Connexin-26 antibody at a dilution of 1:400 (red) and Hoechst 33342 (blue). (C) Merge of the two images.Application
WB analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) liver membranes using Connexin-26 antibody at a dilution of 1:200 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.| Product Name | Connexin 26 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Connexin 26forms gap junctions; may play a role in epididymal development [RGD, Feb 2006] |
| Immunogen | Peptide (C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26 (Accession P21994). Intracellular, cytoplasmic loop. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | gap junction protein beta 2 CXN-26 Gjb2 Cx26 |
|---|---|
| Target | Connexin 26 |
Application
ICC/IF analysis of HepG2 cells using Connexin-26 antibody at a dilution of 1:400 (red) and Hoechst 33342 (blue). (C) Merge of the two images.
Application
WB analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) liver membranes using Connexin-26 antibody at a dilution of 1:200 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Reconstituted antibody containsA?phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
