Application
Human Heart stained with anti-Connexin-43 antibodyApplication
staining GJA1 in human heart by Immunohistochemistry (FFPE-sections).| Product Name | Connexin 43 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to GJA1GJA1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5. |
| Immunogen | A synthetic peptide corresponding to a segment of the 3rd cytoplasmic domain of rat GJA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| IHC-P: Use at a dilution of 1/50 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. WB: Use at a concentration of 2 - 4 μg/ml for 2 hours at RT. Predicted molecular weight: 42 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Gja1 Cx43 MGC93610 24392 |
|---|---|
| Target | GJA1 |
| Cellular Localization | Cytoplasmic and Integral membrane protein |
Application
Human Heart stained with anti-Connexin-43 antibody
Application
staining GJA1 in human heart by Immunohistochemistry (FFPE-sections).| Positive Control | Heart |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/50 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. WB: Use at a concentration of 2 - 4 μg/ml for 2 hours at RT. Predicted molecular weight: 42 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
