Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Contactin 1 antibody diluted at 1:1000.Application
Contactin 1 antibody detects Contactin 1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Contactin 1 protein stained by Contactin 1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .| Product Name | Contactin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Contactin 1Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Contactin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Rhesus Monkey, BovineHuman (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AW495098 antibody CNTN antibody Cntn1 antibody F3cam antibody |
|---|---|
| Target | Contactin 1 |
| Molecular Weight(MW) | 113 kDa (note) (3) |
| Cellular Localization | Cell membrane , GPI-anchor |
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Contactin 1 antibody diluted at 1:1000.
Application
Contactin 1 antibody detects Contactin 1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Contactin 1 protein stained by Contactin 1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
