Application
ApplicationApplication
at a 1/1000 dilution staining ~ 105 kDa beta COP in melanoma cell lysate by Western blot.| Product Name | COPB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [maD] to COPBThis gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 701-715 of COPB. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | maD |
| Host Species | Mouse |
| Tested Applications | AFICC/IFIHC-FIHC-PWB |
| AF: Use at an assay dependant dilution. IHC-F: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/80 using methanol/acetone or paraformaldehyde fixation, followed by a permeabilization step. Strong immunofluorescent staining is confined mainly to the central perinuclear area in the cell. IHC: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. May be used for micro-injection into cells. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMonkeyRatHamster |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 600959 beta COP P53618 DKFZp761K102 COPB 1315 FLJ10341 FLJ46444 FLJ57957 |
|---|---|
| Target | COPB |
| Tissue Specificity | Recognises an epitope in the COPB protein (110kD). The antibody stains the periphery of the Golgi complex in immunohistology using cultured cells. |
Application
Application
Application
at a 1/1000 dilution staining ~ 105 kDa beta COP in melanoma cell lysate by Western blot.| Application Notes | AF: Use at an assay dependant dilution. IHC-F: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/80 using methanol/acetone or paraformaldehyde fixation, followed by a permeabilization step. Strong immunofluorescent staining is confined mainly to the central perinuclear area in the cell. IHC: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. May be used for micro-injection into cells. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: Ascites fluid |
