Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Copine I antibody at 1:100 dilution.Application
Copine I antibody [N3C3] detects Copine I protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Copine I antibody [N3C3] dilution: 1:500| Product Name | Copine 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Copine 1 (copine I)Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Copine 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rabbit, Sheep, Rhesus Monkey, BovineMouse (93%), Rat (93%), Dog (93%), Pig (96%), Rabbit (95%), Sheep (95%), Rhesus Monkey (99%), Bovine (95%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | COPN1 antibody, CPN1 antibody, MGC1142 antibody, CPNE1 antibody, copine-1 antibody, copine I antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (6) |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Copine I antibody at 1:100 dilution.
Application
Copine I antibody [N3C3] detects Copine I protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Copine I antibody [N3C3] dilution: 1:500| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
