Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using COPS3 antibody at 1:250 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using COPS3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:3000| Product Name | COPS3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COPS3 (COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis))The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COPS3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, BovineMouse (99%), Rat (100%), Zebrafish (94%), Xenopus laevis (94%), Pig (97%), Chicken (98%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CSN3 antibody, SGN3 antibody, COPS3 antibody, JAB1-containing signalosome subunit 3 antibody, COP9 complex subunit 3 antibody, signalosome subunit 3 antibody, COP9 signalosome complex subunit 3 antibody, COP9 constitutive photomorphogenic homolog subunit 3 |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (7) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using COPS3 antibody at 1:250 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using COPS3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:3000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
