Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COPS4 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using COPS4 antibody at 1:100 dilution.| Product Name | COPS4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COPS4 (COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis))This gene encodes one of eight subunits composing COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COPS4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (100%), Rat (100%), Zebrafish (97%), Xenopus laevis (97%), Pig (100%), Chicken (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSN4 antibody, MGC10899 antibody, MGC15160 antibody, COPS4 antibody, COP9 signalosome complex subunit 4 antibody, JAB1-containing signalosome subunit 4 antibody, SGN4 antibody, signalosome subunit 4 antibody, COP9 constitutive photomorphogenic homolog subu |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COPS4 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using COPS4 antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
