| Product Name | Corticosterone antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CorticosteroneCorticosterone is a steroid hormone secreted by the outer layer, or cortex, of the adrenal gland . Classed as a glucocorticoid, corticosterone helps regulate the conversion of amino acids into carbohydrates and glycogen by the liver, and helps stimulate glycogen formation in the tissues. Corticosterone is similar in structure, although somewhat less potent, than the other glucocorticoids cortisol and cortisone . It is produced in response to stimulation by the pituitary substance adrenocorticotropic hormone (ACTH). In some species, but not in humans, corticosterone is the predominant glucocorticoid secreted by the adrenal. It is a precursor in the synthesis of aldosterone , another adrenal cortical steroid. Corticosterone Levels1. Men 420 ± 220 pg/0.1 ml2. Women Follicular Phase 240 ± 150 pg/0.1 ml Luteal Phase 440 ± 230 pg/0.1 ml3. Laying Hens 220 ± 50 pg/0.1 ml |
| Immunogen | Corticosterone-21-thyroglobulin used as the immunogen. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | RIA |
| For stock solution: add 5ml of dilution buffer (0.05M Tris-HCl buffer, pH 8.0, containing 0.1 sodium chloride, 0.1% bovine serum albumin, and 0.1% sodium azide). Rotate vial gently until powder is dissolved.RIA: The antiserum is characterized utilizing the following dextran coated charcoal radioimmunoassay (RIA) protocol, where 0.5 ml of reconstituted and diluted antiserum has been found to bind at least 40% of 15 picograms of tritiated (3H) corticosterone with a specific activity of approximately 100 ci/mmole.RIA recommended protocol can be supplied upon request - please send an email to support:genetex.com for further details. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Corticosterone |
|---|---|
| Tissue Specificity | Specificity of the antiserum is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding. The cross-reactivity data obtained in the described-RIA system is as follows:Please refer to additional information for results.Cross-Reactant %Cross-ReactivityDehydroepiandrosterone 11-Deoxycorticosterone 5-á-Dihydrotestosterone 17-Hydroxyprogesterone 20á-Hydroxyprogesterone 20â-Hydroxyprogesterone |
| Application Notes | For stock solution: add 5ml of dilution buffer (0.05M Tris-HCl buffer, pH 8.0, containing 0.1 sodium chloride, 0.1% bovine serum albumin, and 0.1% sodium azide). Rotate vial gently until powder is dissolved.RIA: The antiserum is characterized utilizing the following dextran coated charcoal radioimmunoassay (RIA) protocol, where 0.5 ml of reconstituted and diluted antiserum has been found to bind at least 40% of 15 picograms of tritiated (3H) corticosterone with a specific activity of approximately 100 ci/mmole.RIA recommended protocol can be supplied upon request - please send an email to support:genetex.com for further details. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Prior to reconstitution store at 4°C. Following reconstitution keep as concentrated solution, aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. Avoid freeze-thaw cycles. |
| Storage Buffer | Whole serum containing no preservatives |
