Application
Western Blot analysis of NR2F1 expression in transfected 293T cell line by NR2F1 polyclonal antibody. A: Non-transfected lysate. B: NR2F1 transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COUP TF1 antibody at 1:200 dilution.Application
COUP TF1 antibody [N1], N-term detects COUP TF1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. COUP TF1 antibody [N1], N-term diluted at 1:500.| Product Name | COUP TF1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COUP TF1 (nuclear receptor subfamily 2, group F, member 1)Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human COUP TF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | COUP-TFI antibody, EAR-3 antibody, EAR3 antibody, ERBAL3 antibody, NR2F2 antibody, SVP44 antibody, TCFCOUP1 antibody, TFCOUP1 antibody, NR2F1 antibody, COUP-TF1 antibody, V-erbA-related protein 3 antibody, COUP transcription factor 1 antibody, COUP-TF I an |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) |
Application
Western Blot analysis of NR2F1 expression in transfected 293T cell line by NR2F1 polyclonal antibody. A: Non-transfected lysate. B: NR2F1 transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COUP TF1 antibody at 1:200 dilution.
Application
COUP TF1 antibody [N1], N-term detects COUP TF1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. COUP TF1 antibody [N1], N-term diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
