Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000Application
COX1 antibody detects COX1 protein by western blot analysis. A. 50 μg mouse brain extract 7.5 % SDS-PAGE COX1 antibody dilution: 1:1000Application
COX1 antibody detects PTGS1 protein at cytosol on by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. COX1 antibody dilution: 1:500.Application
COX1 antibody detects COX1 protein by western blot analysis. A. 50 μg rat brain extract 7.5 % SDS-PAGE COX1 antibody dilution: 1:1000| Product Name | COX1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COX1 (prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase))Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COX1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Rabbit, Sheep, BovineDog (92%), Rabbit (93%), Sheep (93%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | COX1 antibody, COX3 antibody, PCOX1 antibody, PGG/HS antibody, PGHS-1 antibody, PGHS1 antibody, PHS1 antibody, PTGHS antibody, PTGS1 antibody, prostaglandin G/H synthase 1 antibody, cyclooxygenase-1 antibody, PGH synthase 1 antibody, COX-1 antibody, prosta |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) (8) |
| Cellular Localization | Microsome membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein |
Application
Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000
Application
COX1 antibody detects COX1 protein by western blot analysis. A. 50 μg mouse brain extract 7.5 % SDS-PAGE COX1 antibody dilution: 1:1000
Application
COX1 antibody detects PTGS1 protein at cytosol on by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. COX1 antibody dilution: 1:500.
Application
COX1 antibody detects COX1 protein by western blot analysis. A. 50 μg rat brain extract 7.5 % SDS-PAGE COX1 antibody dilution: 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
