Application
Immunohistochemistry (IHC) analyzes of Cox-2 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.Application
Western blot (WB) analysis of Cox-2 pAb at 1:500 dilution. Lane1:Hela whole cell lysate treated with LPS. Lane2:Jurkat whole cell lysate treated with LPS. Lane3:Raw264.7 whole cell lysate treated with LPS. Lane3:Rat lung tissue lysate| Product Name | COX2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COX2Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009] |
| Immunogen | Synthetic peptide, corresponding to amino acids C-terminus of Human Cox-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500-1:1000, IHC: 1:50-1:200, IF: 1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PTGS2 , hCox2 , PHS2 , PGG/HS , PGHS2 , prostaglandin-endoperoxide synthase 2 , GRIPGHS |
|---|---|
| Target | COX2 |
| Molecular Weight(MW) | 74 kDa (note) |
| Tissue Specificity | Cox2 (T594) pAb detects endogenous levels of Cox2 protein. |
Application
Immunohistochemistry (IHC) analyzes of Cox-2 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Application
Western blot (WB) analysis of Cox-2 pAb at 1:500 dilution. Lane1:Hela whole cell lysate treated with LPS. Lane2:Jurkat whole cell lysate treated with LPS. Lane3:Raw264.7 whole cell lysate treated with LPS. Lane3:Rat lung tissue lysate| Application Notes | WB: 1:500-1:1000, IHC: 1:50-1:200, IF: 1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
