Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using COX2 antibody at 1:100 dilution.Application
Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | COX2 antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COX2 (prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase))Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Cat, Pig, Sheep, BovineDog (93%), Cat (93%), Pig (93%), Sheep (93%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | COX-2 antibody, COX2 antibody, GRIPGHS antibody, PGG/HS antibody, PGHS-2 antibody, PHS-2 antibody, hCox-2 antibody, PTGS2 antibody, prostaglandin H2 synthase 2 antibody, cyclooxygenase 2b antibody, prostaglandin G/H synthase 2 antibody, PGH synthase 2 anti |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using COX2 antibody at 1:100 dilution.
Application
Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
