Application
Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE COX6B1 antibody diluted at 1:1000Application
COX6B1 antibody [N2C3] detects COX6B1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COX6B1 protein stained by COX6B1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded BCC3 xenograft, using COX6B1 antibody at 1:100 dilution.| Product Name | COX6B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COX6B1 (cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous))Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIb. Three pseudogenes COX6BP-1, COX6BP-2 and COX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2, respectively. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX6B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | COX6B antibody, COXG antibody, COXVIb1 antibody, COX6B1 antibody, cytochrome c oxidase subunit 6B1 antibody, COX VIb-1 antibody, cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) antibody |
|---|---|
| Molecular Weight(MW) | 10 kDa (note) |
| Cellular Localization | Mitochondrion intermembrane space |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE COX6B1 antibody diluted at 1:1000
Application
COX6B1 antibody [N2C3] detects COX6B1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COX6B1 protein stained by COX6B1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded BCC3 xenograft, using COX6B1 antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
