Application
Sample (30 µg of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using CAR antibody at 1:500 dilution.Application
Immunoprecipitation of CAR protein from HeLa whole cell extracts using 5 μg of CAR antibody . Western blot analysis was performed using CAR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | Coxsackie Adenovirus Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Coxsackie Adenovirus Receptor (coxsackie virus and adenovirus receptor)The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Coxsackie Adenovirus Receptor. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Rabbit, BovineMouse (90%), Rabbit (90%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CAR antibody, CAR4/6 antibody, HCAR antibody, CXADR antibody, 46 kD coxsackievirus and adenovirus receptor (CAR) protein antibody, coxsackievirus and adenovirus receptor antibody, CVB3-binding protein antibody, HCVADR antibody, coxsackievirus B-adenovirus |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (3) |
| Cellular Localization | Isoform 1: Cell membrane , Cell junction , tight junction , adherens junction , Basolateral cell membrane , Isoform 2: Cell membrane , Basolateral cell membrane , Isoform 3: Secreted , Isoform 4: Secreted , Isoform 5: Secreted |
Application
Sample (30 µg of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using CAR antibody at 1:500 dilution.
Application
Immunoprecipitation of CAR protein from HeLa whole cell extracts using 5 μg of CAR antibody . Western blot analysis was performed using CAR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
