Application
An ELISA plate is coated with 50 μL of a Proinsulin recombinant protein at concentration ranged from 0.2 μg/mL to 1.6 μg/mL. The coated protein is detected with anti-C-peptide antibody at 500 ng/mL.| Product Name | C-peptide antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C-peptide (insulin)After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human insulin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IRDN , MODY10 , IDDM2 , INS , ILPR , insulin |
|---|---|
| Target | C-peptide |
| Molecular Weight(MW) | 12 kDa (note) (4) |
| Cellular Localization | Secreted |
Application
An ELISA plate is coated with 50 μL of a Proinsulin recombinant protein at concentration ranged from 0.2 μg/mL to 1.6 μg/mL. The coated protein is detected with anti-C-peptide antibody at 500 ng/mL.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
