| Product Name | C-Peptide antibody [4H8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4H8] to C-PeptideC-Peptide is part of the molecule of Proinsulin, that consists of three parts: C-Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C-Peptide are produced C-peptide is released into the bloodstream in equal amounts to insulin. A test of C-peptide levels will show how much insulin the body is making. |
| Immunogen | Recombinant fragment |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4H8 |
| Host Species | Mouse |
| Tested Applications | ELISARIA |
| ELISA: Use at an assay dependent dilution. RIA: Use at an assay dependent dilution. Competitive ELISA or RIA detection of C-peptide in human serum after immunospecific removal of proinsulin (with solid-phased Mabs to human proinsulin). Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assay for the measurement of total human proinsulin (including proinsulin convertion intermediates) in serum. Specific immunosorbtional removal of total proinsulin and C-peptide molecules from serum samples (with solid-phase of this antibody) before the measurement of human insulin. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | P01308, 3630, C Peptide, 125850, INS, IDDM2, ILPR, IRDN, MODY10 |
|---|---|
| Target | C-Peptide |
| Tissue Specificity | This antibody binds with equal efficiency to the human free C-peptide and C-peptide region in proinsulin molecules. Antigenic determinants identified by the antibody are in the three-dimensional structure generated by N- and C-terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.Affinity (Kd) > 1 x 108.This antibody does not cross-react with human, bovine, porcine and mouse/rat insulin. |
| Cellular Localization | Secreted |
| Light Chain Type | kappa |
| Application Notes | ELISA: Use at an assay dependent dilution. RIA: Use at an assay dependent dilution. Competitive ELISA or RIA detection of C-peptide in human serum after immunospecific removal of proinsulin (with solid-phased Mabs to human proinsulin). Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assay for the measurement of total human proinsulin (including proinsulin convertion intermediates) in serum. Specific immunosorbtional removal of total proinsulin and C-peptide molecules from serum samples (with solid-phase of this antibody) before the measurement of human insulin. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |
| Storage Buffer | Phosphate buffered saline, pH 7.4, containing 0.1% sodium azide |
