Application
Western blot analysis of human HeLa (heat shocked) cell lysate, probed with Cpn10 (Hsp10) pAb.| Product Name | Cpn10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cpn10Homologs of E. coli GroES make up a family of small heat shock proteins with an approximate molecular mass of 10 kDa (Hsp10s). Cpn10 acts as a cochaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, Cpn10 is thought to interact with cHsp60, a reported immunopathological antigen, identifying cpn10 as a potential contributor to an immunological response. |
| Immunogen | Synthetic peptide: RDGDILGKYVD conjugated to KLH, corresponding to amino acids 91-101 of Human Cpn10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPRIAWB |
| IHC: Use at a dilution of 1/400. IP: Use at a dilution of 1/100. RIA: Use at a dilution of 1/100000. WB (colorimetric): Use at a dilution of 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 9.9 kDa). WB (ELC): Use at a dilution of 1/5000. Detects a band of approximately 10 kDa (predicted molecular weight: 9.9 kDa). Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseDogPigRatGuinea PigRabbitSheepXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cpn10, HSPE1, 3336, GROES, HSP10, 600141, P61604 |
|---|---|
| Target | Cpn10 |
| Cellular Localization | Mitochondrial |
Application
Western blot analysis of human HeLa (heat shocked) cell lysate, probed with Cpn10 (Hsp10) pAb.| Application Notes | IHC: Use at a dilution of 1/400. IP: Use at a dilution of 1/100. RIA: Use at a dilution of 1/100000. WB (colorimetric): Use at a dilution of 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 9.9 kDa). WB (ELC): Use at a dilution of 1/5000. Detects a band of approximately 10 kDa (predicted molecular weight: 9.9 kDa). Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
