Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000Application
CPSF2 antibody [N1N3] detects CPSF2 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CPSF2 protein stained by CPSF2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | CPSF2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CPSF2 (cleavage and polyadenylation specific factor 2, 100kDa)Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CPSF2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (96%), Xenopus laevis (98%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CPSF100 antibody KIAA1367 antibody CPSF2 antibody CPSF 100 kDa subunit antibody cleavage and polyadenylation specificity factor 100 kDa subunit antibody CPSF 100kDa subunit antibody cleavage and polyadenylation specificity factor subunit 2 antibody |
|---|---|
| Molecular Weight(MW) | 88 kDa (note) (4) |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000
Application
CPSF2 antibody [N1N3] detects CPSF2 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CPSF2 protein stained by CPSF2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | NT2D1 , U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
