Rabbit Polyclonal antibody to CPSF73 (cleavage and polyadenylation specificity factor subunit 3)Most eukaryotic mRNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3-prime end. CPSF3 encodes the 73-kD subunit of the cleavage and polyadenylation specificity factor (CPSF) and is the pre-mRNA 3-prime-end-processing endonuclease (Mandel et al., 2006 [PubMed 17128255]).[supplied by OMIM]
Immunogen
Recombinant protein encompassing a sequence within the center region of human CPSF73. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFWB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Chimpanzee, BovineMouse (97%), Rat (97%), Chimpanzee (99%), Bovine (98%)
Concentration
0.5mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
CPSF antibody CPSF-73 antibody CPSF73 antibody YSH1 antibody CPSF3 antibody mRNA 3'-end-processing endonuclease CPSF-73 antibody cleavage and polyadenylation specificity factor subunit 3 antibody cleavage and polyadenylation specificity factor 3 ant
Molecular Weight(MW)
77 kDa (note) (6)
Cellular Localization
Nucleus
Application
Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CPSF73 antibody at 1:500 dilution.
Positive Control
NT2D1
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.