Application
Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] diluted at 1:1000.Application
Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE at 1:1000Application
Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using CRABP2 antibody at 1:500 dilution.Application
CRABP2 antibody [N1C3] detects CRABP2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CRABP2 protein stained by CRABP2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | CRABP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CRABP2 (cellular retinoic acid binding protein 2)A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The inducibility of the CRABP2 gene suggests that this isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CRABP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Rhesus Monkey, BovineXenopus laevis (89%), Pig (95%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CRABP-II antibody, RBP6 antibody, CRABP2 antibody, cellular retinoic acid-binding protein 2 antibody, cellular retinoic acid-binding protein II antibody, cellular retinoic acid binding protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 16 kDa (note) (2) |
Application
Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using CRABP2 antibody at 1:500 dilution.
Application
CRABP2 antibody [N1C3] detects CRABP2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CRABP2 protein stained by CRABP2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
