Application
Immunofluorescence analysis of methanol-fixed H1299, using CRBP I antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 15% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CRBP I antibody detects RBP1 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE CRBP I antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human colon, using CRBP I antibody at 1:100 dilution.| Product Name | CRBP I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CRBP I (retinol binding protein 1, cellular)This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CRBP I. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rat, BovineRat (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CRABP-I antibody, CRBP antibody, CRBP1 antibody, CRBPI antibody, RBPC antibody, RBP1 antibody, CRBP-I antibody, cellular retinol-binding protein I antibody, retinol-binding protein 1 antibody, retinol-binding protein 1, cellular antibody, retinol binding p |
|---|---|
| Molecular Weight(MW) | 16 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed H1299, using CRBP I antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 15% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CRBP I antibody detects RBP1 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE CRBP I antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human colon, using CRBP I antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
