Rabbit polyclonal antibody to CREB (phospho Ser129)This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine 129 (I -L-SP-R-R).
Modification
p-Ser129
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-PWB
WB: 1:500-1:1000 IHC: 1:50-1:100
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
CREB , CREB1 , cAMP responsive element binding protein 1
Target
CREB (phospho Ser129)
Tissue Specificity
CREB (phospho-Ser129) antibody detects endogenous levels of CREB only when phosphorylated at serine 129.
Application
Application
IHC-P analysis of human breast carcinoma using CREB (phospho Ser129) antibody.
Application
WB analysis of lysates from untreated and UV-treated 293 cells uding CREB (phospho Ser129) antibody.
Application Notes
WB: 1:500-1:1000 IHC: 1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Storage Buffer
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.