Application
Western blot of a forskolin-stimulated rat hippocampal slice showing phospho-specific immunolabeling of the ~45k CREB protein phosphorylated at Ser 133 using CREB (phospho Ser133) antibody .| Product Name | CREB (phospho Ser133) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CREB (phospho Ser133)This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH |
| Modification | p-Ser133 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cAMP responsive element binding protein 1, CREB, cAMP responsive element binding protein1, MGC9284, CREB1, CREB (phospho S133), CREB (phospho Ser133), CREB (pS133), CREB (pSer133), CREB phospho S133, CREB phospho Ser133, Phospho CREB (S133), Phospho CREB ( |
|---|---|
| Target | CREB (phospho Ser133) |
| Molecular Weight(MW) | 45 (note) |
Application
Western blot of a forskolin-stimulated rat hippocampal slice showing phospho-specific immunolabeling of the ~45k CREB protein phosphorylated at Ser 133 using CREB (phospho Ser133) antibody .| Application Notes | Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
