Application
ICC/IF analysis of MCF7 cells using CREB (phospho Ser133) antibody (red) and DAPI (blue).Application
WB analysis of extracts from untreated and UV-treated HeLa cells using CREB (phospho Ser133) antibody.| Product Name | CREB (phospho Ser133) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CREB (phospho Ser133)This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding Ser133 of human CREB |
| Modification | p-Ser133 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cAMP responsive element binding protein 1 , CREB1 , CREB |
|---|---|
| Target | CREB (phospho Ser133) |
| Molecular Weight(MW) | 43 kDa (note) |
Application
ICC/IF analysis of MCF7 cells using CREB (phospho Ser133) antibody (red) and DAPI (blue).
Application
WB analysis of extracts from untreated and UV-treated HeLa cells using CREB (phospho Ser133) antibody.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
