Application
Western blot analysis of extracts from Hela cells untreated or treated with UV using CREB(Phospho-Ser133)Antibody.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser133) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | CREB (phospho Ser133) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CREB (Phospho Ser133)CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories . |
| Immunogen | Peptide sequence around phosphorylation site of serine 133 (R-P-S(p)-Y-R) derived from Human CREB. |
| Modification | p-Ser133 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CREB, MGC9284, CREB1, cyclic AMP-responsive element-binding protein 1, active transcription factor CREB, transactivator protein, CREB-1, cAMP-response element-binding protein-1, cAMP-responsive element-binding protein 1, cAMP responsive element binding pro |
|---|---|
| Target | CREB (Phospho Ser133) |
| Molecular Weight(MW) | 43 (note) |
Application
Western blot analysis of extracts from Hela cells untreated or treated with UV using CREB(Phospho-Ser133)Antibody.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser133) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
