Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody diluted at 1:1000.Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced. (PDGF: Platelet-derived growth factor)Application
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.| Product Name | CREB (phospho Ser133) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CREB (phospho Ser133) (cAMP responsive element binding protein 1)This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human CREB (phospho Ser133). The exact sequence is proprietary. |
| Modification | p-Ser133 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineRat (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | CREB (phospho Ser133) |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody diluted at 1:1000.
Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced. (PDGF: Platelet-derived growth factor)
Application
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
