Application
Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE CREM antibody diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CREM antibody at 1:200 dilution.Application
CREM antibody detects CREM protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE CREM antibody dilution: 1:1000Application
CREM antibody detects CREM protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract 12 % SDS-PAGE CREM antibody dilution: 1:1000| Product Name | CREM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CREM (cAMP responsive element modulator)This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CREM. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Chicken, BovineDog (91%), Chicken (87%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ICER antibody MGC111110 antibody MGC17881 antibody MGC41893 antibody hCREM-2 antibody CREM antibody cAMP-responsive element modulator antibody inducible cAMP early repressor ICER antibody CREM 2alpha-b protein antibody cAMP response element modula |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE CREM antibody diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CREM antibody at 1:200 dilution.
Application
CREM antibody detects CREM protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE CREM antibody dilution: 1:1000
Application
CREM antibody detects CREM protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract 12 % SDS-PAGE CREM antibody dilution: 1:1000| Positive Control | NT2D1 , Neuro2A , GL261 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
