Application
ELISA analysis of control antigen and antigen using CRK antibody [3G11E8].Application
WB analysis of human CRK (a.a. 1-204) recombinant protein using CRK antibody [3G11E8].Application
IHC-P analysis of lung cancer tissues using CRK antibody [3G11E8].Application
WB analysis of lysates from (1) untransfected and (2) CRK (a.a. 1-204)-hIgGFc transfected HEK293 cells using CRK antibody [3G11E8].Application
IHC-P analysis of intima cancer tissues using CRK antibody [3G11E8].Application
ICC/IF analysis of 3T3-L1 cells using CRK antibody [3G11E8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
FACS analysis of HeLa cells using CRK antibody [3G11E8] (blue) or a negative control (red).| Product Name | CRK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [3G11E8] to CRKThis gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human CRK expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3G11E8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CRKII P38 |
|---|---|
| Target | CRK |
| Molecular Weight(MW) | 42 kDa (note) |
Application
ELISA analysis of control antigen and antigen using CRK antibody [3G11E8].
Application
WB analysis of human CRK (a.a. 1-204) recombinant protein using CRK antibody [3G11E8].
Application
IHC-P analysis of lung cancer tissues using CRK antibody [3G11E8].
Application
WB analysis of lysates from (1) untransfected and (2) CRK (a.a. 1-204)-hIgGFc transfected HEK293 cells using CRK antibody [3G11E8].
Application
IHC-P analysis of intima cancer tissues using CRK antibody [3G11E8].
Application
ICC/IF analysis of 3T3-L1 cells using CRK antibody [3G11E8] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
FACS analysis of HeLa cells using CRK antibody [3G11E8] (blue) or a negative control (red).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
