Application
Immunofluorescence analysis of methanol-fixed A431, using CRK antibody at 1:200 dilution.Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3] diluted at 1:1000 and competitor's antibody (SC-289) diluted by 1:200.Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE diluted at 1:1000| Product Name | CRK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CRK (v-crk sarcoma virus CT10 oncogene homolog (avian))This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CRK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Chicken, BovineRat (99%), Xenopus laevis (87%), Pig (98%), Chicken (91%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CRKII antibody, CRK antibody, adapter molecule crk antibody, proto-oncogene c-Crk antibody, v-crk sarcoma virus CT10 oncogene homolog (avian) antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed A431, using CRK antibody at 1:200 dilution.
Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3] diluted at 1:1000 and competitor's antibody (SC-289) diluted by 1:200.
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
