Application
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CrkL antibody detects CrkL protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. CrkL antibody dilution: 1:500.Application
CrkL antibody detects CrkL protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. CrkL antibody dilution: 1:500.Application
CrkL antibody detects CrkL protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CrkL protein stained by CrkL antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunoprecipitation of CrkL protein from 293T whole cell extracts using 5 μg of CrkL antibody . Western blot analysis was performed using CrkL antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | CrkL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CrkL (v-crk sarcoma virus CT10 oncogene homolog (avian)-like)This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CrkL. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Rhesus MonkeyRat (96%), Zebrafish (83%), Xenopus laevis (87%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CRKL antibody, crk-like protein antibody, v-crk sarcoma virus CT10 oncogene homolog (avian)-like antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (2) |
Application
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with CrkL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CrkL antibody detects CrkL protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. CrkL antibody dilution: 1:500.
Application
CrkL antibody detects CrkL protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. CrkL antibody dilution: 1:500.
Application
CrkL antibody detects CrkL protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CrkL protein stained by CrkL antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunoprecipitation of CrkL protein from 293T whole cell extracts using 5 μg of CrkL antibody . Western blot analysis was performed using CrkL antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
