Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE COT antibody diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CROT antibody at 1:100 dilution.| Product Name | CROT antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CROT (carnitine O-octanoyltransferase)This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CROT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | PigPig (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | COT antibody, CROT antibody, peroxisomal carnitine acyltransferase antibody, peroxisomal carnitine O-octanoyltransferase antibody, carnitine O-octanoyltransferase antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (2) |
| Cellular Localization | Peroxisome |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE COT antibody diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CROT antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
