Application
Sample(30 µg of whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:1500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Cryopyrin antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Cryopyrin (NLR family, pyrin domain containing 3)This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cryopyrin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (85%), Rat (92%), Pig (92%), Rhesus Monkey (92%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AGTAVPRL antibody AII antibody AII/AVP antibody AVP antibody C1orf7 antibody CIAS1 antibody CLR1.1 antibody FCAS antibody FCU antibody FLJ95925 antibody MWS antibody NALP3 antibody PYPAF1 antibody NLRP3 antibody cold autoinflammatory syndrome |
|---|---|
| Target | Cryopyrin |
| Molecular Weight(MW) | 118 kDa (note) (6) |
| Cellular Localization | Cytoplasm |
Application
Sample(30 µg of whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:1500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
