Application
Immunofluorescence analysis of methanol-fixed HeLa, using CSDE1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using CSDE1 antibody at 1:250 dilution.Application
CSDE1 antibody [N2C1], Internal detects CSDE1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE CSDE1 antibody [N2C1], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CSDE1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CSDE1 (cold shock domain containing E1, RNA-binding)RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CSDE1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Chicken, BovineMouse (99%), Rat (99%), Zebrafish (83%), Chicken (96%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | D1S155E antibody, DKFZp779B0247 antibody, DKFZp779J1455 antibody, FLJ26882 antibody, RP5-1000E10.3 antibody, UNR antibody, CSDE1 antibody, upstream of NRAS antibody, N-ras upstream gene protein antibody, cold shock domain-containing protein E1 antibody, NR |
|---|---|
| Molecular Weight(MW) | 89 kDa (note) (7) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using CSDE1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using CSDE1 antibody at 1:250 dilution.
Application
CSDE1 antibody [N2C1], Internal detects CSDE1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE CSDE1 antibody [N2C1], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
