Application
Sample (30μg whole cell lysate) A:293T B:H1299 C:MOLT4 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using c-Src antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded HT29 xenograft , using c-Src antibody at 1:100 dilution.| Product Name | CSK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to c-Src (c-src tyrosine kinase)Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CSK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Pig, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (99%), Zebrafish (88%), Pig (99%), Chicken (95%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC117393 antibody, CSK antibody, protein-tyrosine kinase CYL antibody, tyrosine-protein kinase CSK antibody, C-Src kinase antibody, c-src tyrosine kinase antibody |
|---|---|
| Target | c-Src |
| Molecular Weight(MW) | 51 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Cell membrane |
Application
Sample (30μg whole cell lysate) A:293T B:H1299 C:MOLT4 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using c-Src antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded HT29 xenograft , using c-Src antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
