Application
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using Cleavage stimulation factor 2 antibody at 1:500 dilution.Application
CSTF2 antibody [N1N2], N-term detects CSTF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CSTF2 protein stained by CSTF2 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000| Product Name | CSTF2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CSTF2 (cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa)This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CSTF2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, BovineMouse (99%), Rat (99%), Zebrafish (94%), Xenopus laevis (96%), Chicken (95%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CstF-64 antibody, CSTF2 antibody, cleavage stimulation factor 64 kDa subunit antibody, betaCstF-64 variant 2 antibody, CF-1 64 kDa subunit antibody, cleavage stimulation factor subunit 2 antibody, CSTF 64 kDa subunit antibody, cleavage stimulation factor, |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using Cleavage stimulation factor 2 antibody at 1:500 dilution.
Application
CSTF2 antibody [N1N2], N-term detects CSTF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CSTF2 protein stained by CSTF2 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
