Application
Western blot analysis of extracts from Jurkat cells treated with TNF using CtBP1 (Phospho-Ser422) Antibody. The lane on the right is treated with the antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | CtBP1 (phospho Ser422) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CtBP1 (phospho Ser422)This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of Serine 422(A-P-S(p)-P-G) derived from Human CtBP1. |
| Modification | p-Ser422 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CTBP1 , BARS , C-terminal binding protein 1 |
|---|---|
| Target | CtBP1 (phospho Ser422) |
| Tissue Specificity | The antibody detects endogenous levels of CtBP1 only when phosphorylated at serine 422. |
Application
Western blot analysis of extracts from Jurkat cells treated with TNF using CtBP1 (Phospho-Ser422) Antibody. The lane on the right is treated with the antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
